Ontology highlight
ABSTRACT:
SUBMITTER: Jamalian A
PROVIDER: S-EPMC4223500 | biostudies-literature | 2014 Nov
REPOSITORIES: biostudies-literature
Jamalian Azadeh A Sneekes Evert-Jan EJ Wienk Hans H Dekker Lennard J M LJ Ruttink Paul J A PJ Ursem Mario M Luider Theo M TM Burgers Peter C PC
Molecular & cellular proteomics : MCP 20140714 11
Here we describe a new method to identify calcium-binding sites in proteins using high-resolution liquid chromatography-mass spectrometry in concert with calcium-directed collision-induced dissociations. Our method does not require any modifications to the liquid chromatography-mass spectrometry apparatus, uses standard digestion protocols, and can be applied to existing high-resolution MS data files. In contrast to NMR, our method is applicable to very small amounts of complex protein mixtures ...[more]