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Isotropic 3D Super-resolution Imaging with a Self-bending Point Spread Function.

ABSTRACT: Airy beams maintain their intensity profiles over a large propagation distance without substantial diffraction and exhibit lateral bending during propagation1-5. This unique property has been exploited for micromanipulation of particles6, generation of plasma channels7 and guidance of plasmonic waves8, but has not been explored for high-resolution optical microscopy. Here, we introduce a self-bending point spread function (SB-PSF) based on Airy beams for three-dimensional (3D) super-resolution fluorescence imaging. We designed a side-lobe-free SB-PSF and implemented a two-channel detection scheme to enable unambiguous 3D localization of fluorescent molecules. The lack of diffraction and the propagation-dependent lateral bending make the SB-PSF well suited for precise 3D localization of molecules over a large imaging depth. Using this method, we obtained super-resolution imaging with isotropic 3D localization precision of 10-15 nm over a 3 ?m imaging depth from ?2000 photons per localization.

SUBMITTER: Jia S

PROVIDER: S-EPMC4224117 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature

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Isotropic 3D Super-resolution Imaging with a Self-bending Point Spread Function.

Jia Shu S Vaughan Joshua C JC Zhuang Xiaowei X

Nature photonics 20140101

Airy beams maintain their intensity profiles over a large propagation distance without substantial diffraction and exhibit lateral bending during propagation1-5. This unique property has been exploited for micromanipulation of particles6, generation of plasma channels7 and guidance of plasmonic waves8, but has not been explored for high-resolution optical microscopy. Here, we introduce a self-bending point spread function (SB-PSF) based on Airy beams f ...[more]

PMID: 25383090

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