Ontology highlight
ABSTRACT:
SUBMITTER: Jee JE
PROVIDER: S-EPMC4224607 | biostudies-literature | 2014 Dec
REPOSITORIES: biostudies-literature
Jee Joo-Eun JE Lim Jaehong J Hyun Hoon H Oon Jessica J Ong Yong Siang YS Massif Cedrik C Chang Young-Tae YT Choi Hak Soo HS Lee Su Seong SS
Chemical communications (Cambridge, England) 20141201 96
Screening of bead-based peptide libraries against fluorescent dye-labeled target proteins was found to be significantly influenced by the dye characteristics. Commercially available red fluorescent dyes with net negative charges adversely showed strong interactions with library beads. The introduction of zwitterionic dyes significantly reduced the unwanted interactions, which sheds light upon using the right fluorescent probe for acquisition of reliable results in various fluorescence-assisted a ...[more]