CRISPR transcriptional repression devices and layered circuits in mammalian cells.
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ABSTRACT:
SUBMITTER: Kiani S
PROVIDER: S-EPMC4228775 | biostudies-literature | 2014 Jul
REPOSITORIES: biostudies-literature
Publications
CRISPR transcriptional repression devices and layered circuits in mammalian cells.
Kiani Samira S Beal Jacob J Ebrahimkhani Mohammad R MR Huh Jin J Hall Richard N RN Xie Zhen Z Li Yinqing Y Weiss Ron R
Nature methods 20140505 7
A key obstacle to creating sophisticated genetic circuits has been the lack of scalable device libraries. Here we present a modular transcriptional repression architecture based on clustered regularly interspaced palindromic repeats (CRISPR) system and examine approaches for regulated expression of guide RNAs in human cells. Subsequently we demonstrate that CRISPR regulatory devices can be layered to create functional cascaded circuits, which provide a valuable toolbox for engineering purposes. ...[more]
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