Comparative assessment of fluorescent transgene methods for quantitative imaging in human cells.
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ABSTRACT:
SUBMITTER: Mahen R
PROVIDER: S-EPMC4230620 | biostudies-literature | 2014 Nov
REPOSITORIES: biostudies-literature
Publications
Comparative assessment of fluorescent transgene methods for quantitative imaging in human cells.
Mahen Robert R Koch Birgit B Wachsmuth Malte M Politi Antonio Z AZ Perez-Gonzalez Alexis A Mergenthaler Julia J Cai Yin Y Ellenberg Jan J
Molecular biology of the cell 20140917 22
Fluorescence tagging of proteins is a widely used tool to study protein function and dynamics in live cells. However, the extent to which different mammalian transgene methods faithfully report on the properties of endogenous proteins has not been studied comparatively. Here we use quantitative live-cell imaging and single-molecule spectroscopy to analyze how different transgene systems affect imaging of the functional properties of the mitotic kinase Aurora B. We show that the transgene method ...[more]
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