Ontology highlight
ABSTRACT:
SUBMITTER: Sasaki H
PROVIDER: S-EPMC4231237 | biostudies-literature | 2014 Sep
REPOSITORIES: biostudies-literature
Sasaki Haruka H Yoshida Keita K Hozumi Akiko A Sasakura Yasunori Y
Development, growth & differentiation 20140912 7
Knockout of genes with CRISPR/Cas9 is a newly emerged approach to investigate functions of genes in various organisms. We demonstrate that CRISPR/Cas9 can mutate endogenous genes of the ascidian Ciona intestinalis, a splendid model for elucidating molecular mechanisms for constructing the chordate body plan. Short guide RNA (sgRNA) and Cas9 mRNA, when they are expressed in Ciona embryos by means of microinjection or electroporation of their expression vectors, introduced mutations in the target ...[more]