Integrin expression in esophageal squamous cell carcinoma: loss of the physiological integrin expression pattern correlates with disease progression.

ABSTRACT: The integrins are a family of heterodimeric transmembrane signaling receptors that mediate the adhesive properties of epithelial cells affecting cell growth and differentiation. In many epithelial malignancies, altered integrin expression is associated with tumor progression and often correlates with unfavorable prognosis. However, only few studies have investigated the role of integrin expression in esophageal squamous cell carcinoma (ESCC). Using a novel quantifying immunofluorescence-staining assay, we investigated the expression of the integrins ?2?1, ?3?1, ?6?1, and ?6?4 in primary ESCC of 36 patients who underwent surgical resection. Magnitude and distribution of expression were analyzed in primary tumor samples and autologous esophageal squamous epithelium. The persistence of the physiologically polarized expression of the subunits ?6, ?1, and ?4 in the tumor tissue was significantly associated with prolonged relapse-free survival (p?=?0.028, p?=?0.034, p?=?0.006). In contrast, patients with reduced focal ?6 expression at the tumor invasion front shared a significantly shortened relapse-free survival compared to patients with strong ?6 expression at their stromal surfaces, as it was regularly observed in normal esophageal epithelium (p?=?0.001). Multivariate regression analysis identified the maintenance of strong ?6 immunoreactivity at the invasion front as an independent prognostic factor for increased relapse-free and disease-specific survival (p?=?0.003; p?=?0.003). Our findings suggest that alterations in both pattern and magnitude of integrin expression may play a major role in the disease progression of ESCC patients. Particularly, the distinct expression of the integrins ?6?4 and ?6?1 at the invasion front as well as the maintenance of a polarized integrin expression pattern in the tumor tissue may serve as valuable new markers to assess the aggressiveness of ESCC.

SUBMITTER: Vay C 

PROVIDER: S-EPMC4232252 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature