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A Versatile Multiple Target Detection System Based on DNA Nano-assembled Linear FRET Arrays.

ABSTRACT: DNA molecules have been utilized both as powerful synthetic building blocks to create nanoscale architectures and as inconstant programmable templates for assembly of biosensors. In this paper, a versatile, scalable and multiplex detection system is reported based on an extending fluorescent resonance energy transfer (FRET) cascades on a linear DNA assemblies. Seven combinations of three kinds of targets are successfully detected through the changes of fluorescence spectra because of the three-steps FRET or non-FRET continuity mechanisms. This nano-assembled FRET-based nanowire is extremely significant for the development of rapid, simple and sensitive detection system. The method used here could be extended to a general platform for multiplex detection through more-step FRET process.

SUBMITTER: Li Y

PROVIDER: S-EPMC4882533 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature

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A Versatile Multiple Target Detection System Based on DNA Nano-assembled Linear FRET Arrays.

Li Yansheng Y Du Hongwu H Wang Wenqian W Zhang Peixun P Xu Liping L Wen Yongqiang Y Zhang Xueji X

Scientific reports 20160527

DNA molecules have been utilized both as powerful synthetic building blocks to create nanoscale architectures and as inconstant programmable templates for assembly of biosensors. In this paper, a versatile, scalable and multiplex detection system is reported based on an extending fluorescent resonance energy transfer (FRET) cascades on a linear DNA assemblies. Seven combinations of three kinds of targets are successfully detected through the changes of fluorescence spectra because of the three-s ...[more]

PMID: 27230484

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