Pyrazine-derived disulfide-reducing agent for chemical biology.
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ABSTRACT:
SUBMITTER: Lukesh JC
PROVIDER: S-EPMC4237594 | biostudies-literature | 2014 Aug
REPOSITORIES: biostudies-literature
Publications
Pyrazine-derived disulfide-reducing agent for chemical biology.
Lukesh John C JC Wallin Kelly K KK Raines Ronald T RT
Chemical communications (Cambridge, England) 20140801 67
For fifty years, dithiothreitol (DTT) has been the preferred reagent for the reduction of disulfide bonds in proteins and other biomolecules. Herein we report on the synthesis and characterization of 2,3-bis(mercaptomethyl)pyrazine (BMMP), a readily accessible disulfide-reducing agent with reactivity under biological conditions that is markedly superior to DTT and other known reagents. ...[more]
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