Ontology highlight
ABSTRACT:
SUBMITTER: Zhang X
PROVIDER: S-EPMC4238042 | biostudies-literature | 2014 Nov
REPOSITORIES: biostudies-literature
Zhang Xiaoming X Niu DongDong D Carbonell Alberto A Wang Airong A Lee Angel A Tun Vinnary V Wang Zonghua Z Carrington James C JC Chang Chia-en A CE Jin Hailing H
Nature communications 20141119
Small RNAs (sRNAs) are loaded into ARGONAUTE (AGO) proteins to induce gene silencing. In plants, the 5'-terminal nucleotide is important for sRNA sorting into different AGOs. Here we show that microRNA (miRNA) duplex structure also contributes to miRNA sorting. Base pairing at the 15th nucleotide of a miRNA duplex is important for miRNA sorting in both Arabidopsis AGO1 and AGO2. AGO2 favours miRNA duplexes with no middle mismatches, whereas AGO1 tolerates, or prefers, duplexes with central misma ...[more]