Multiple enzymatic activities of ParB/Srx superfamily mediate sexual conflict among conjugative plasmids.
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ABSTRACT:
SUBMITTER: Maindola P
PROVIDER: S-EPMC4241021 | biostudies-literature | 2014 Oct
REPOSITORIES: biostudies-literature
Publications
Multiple enzymatic activities of ParB/Srx superfamily mediate sexual conflict among conjugative plasmids.
Maindola Priyank P Raina Rahul R Goyal Parveen P Goyal Parveen P Atmakuri Krishnamohan K Ojha Abhishek A Gupta Sourabh S Christie Peter J PJ Iyer Lakshminarayan M LM Aravind L L Arockiasamy Arulandu A
Nature communications 20141031
Conjugative plasmids are typically locked in intergenomic and sexual conflicts with co-resident rivals, whose translocation they block using fertility inhibition factors (FINs). We describe here the first crystal structure of an enigmatic FIN Osa deployed by the proteobacterial plasmid pSa. Osa contains a catalytically active version of the ParB/Sulfiredoxin fold with both ATPase and DNase activity, the latter being regulated by an ATP-dependent switch. Using the Agrobacterium tumefaciens VirB/D ...[more]
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