Ontology highlight
ABSTRACT:
SUBMITTER: Bai X
PROVIDER: S-EPMC4244029 | biostudies-literature | 2014
REPOSITORIES: biostudies-literature
Bai Xi X Hu Hong H Chen Huaping H Wei Quanbin Q Yang Zeshen Z Huang Qianming Q
PloS one 20141125 11
Paenibacillus polymyxa A-8, which secretes β-mannosidase, was isolated from the soil sample under a pine tree located in the "Laoban" mountain region of Sichuan, China. The β-mannosidase gene (MANB) was isolated from P. polymyxa A-8, using primers according to the complete genome. The MANB (2,550 bp) encoding 849 amino acid residues was expressed in Escherichia coli. The specific activities of β-mannosidase produced by P. polymyxa A-8 and E. coli pET30a-MANB were 12 nkat/mg and 635 nkat/mg respe ...[more]