Ontology highlight
ABSTRACT:
SUBMITTER: Jiang W
PROVIDER: S-EPMC4245081 | biostudies-literature | 2014
REPOSITORIES: biostudies-literature
Jiang Wei W Tang Jing-Jie JJ Miao Hong-Hua HH Qu Yu-Xiu YX Qin Jie J Xu Jie J Yang Jinbo J Li Bo-Liang BL Song Bao-Liang BL
PloS one 20141126 11
Somatic cell genetics is a powerful approach for unraveling the regulatory mechanism of cholesterol metabolism. However, it is difficult to identify the mutant gene(s) due to cells are usually mutagenized chemically or physically. To identify important genes controlling cholesterol biosynthesis, an unbiased forward genetics approach named validation-based insertional mutagenesis (VBIM) system was used to isolate and characterize the 25-hydroxycholesterol (25-HC)-resistant and SR-12813-resistant ...[more]