Ontology highlight
ABSTRACT:
SUBMITTER: Blakely BL
PROVIDER: S-EPMC4247985 | biostudies-literature | 2014 Dec
REPOSITORIES: biostudies-literature
Blakely Brandon L BL Dumelin Christoph E CE Trappmann Britta B McGregor Lynn M LM Choi Colin K CK Anthony Peter C PC Duesterberg Van K VK Baker Brendon M BM Block Steven M SM Liu David R DR Chen Christopher S CS
Nature methods 20141012 12
We developed molecular tension probes (TPs) that report traction forces of adherent cells with high spatial resolution, can in principle be linked to virtually any surface, and obviate monitoring deformations of elastic substrates. TPs consist of DNA hairpins conjugated to fluorophore-quencher pairs that unfold and fluoresce when subjected to specific forces. We applied TPs to reveal that cellular traction forces are heterogeneous within focal adhesions and localized at their distal edges. ...[more]