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Expression of ?4, ?v, ?1 and ?3 integrins during the implantation window on blastocyst of a mouse model of polycystic ovarian syndromes.

ABSTRACT: BACKGROUND:It has been hypothesized that blastocyst integrin expression changes can affect the spontaneous miscarriage in polycystic ovarian syndromes (PCOS). OBJECTIVE:In this study, the profile of integrin genes and proteins was investigated on blastocyst of the PCOS experimental mouse model. MATERIALS AND METHODS:30 NMRI female mice were equally divided into 3 groups: control, experimental [PCOS that was injected estradiol valerate (40 mg/kg)]. After 8 weeks, each group was hyper stimulated by PMSG and HCG. Vaginal plaque was checked, and mice were investigated 5 days after the test. Progesterone and estradiol levels were determined; ?4, ?v, ?1 and ?3 integrin genes and protein of blastocysts were examined by real time PCR method and immunohistochemistry, respectively. RESULTS:Estradiol level was significantly increased (p?0.035) in PCOS group. Based on our finding, the ratio of genes' expressions ?v, ?3, ?1 and ?4 in PCOS to control group was 0.479±0.01, 0.5±0.001, 2.7±0.4 and 1.023±0.2 respectively. Genes expression showed a great difference (p?0.001) between ?3, ?1 and ?v in PCOS compared to other groups. ?v and ?3 integrin proteins expressed in all groups but intensity of these proteins in PCOS groups, was lower than other groups. CONCLUSION:Pattern of ?v and ?3 integrins expression on the mouse blastocyst surface has an important effect during the implantation window. This pattern has changed in PCOS model and might have a great influence on implantation failure. Therefore, this experimental study suggests that a great attention to this problem may be essential in patients who are involved.

SUBMITTER: Peyghambari F

PROVIDER: S-EPMC4248147 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature

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Expression of α4, αv, β1 and β3 integrins during the implantation window on blastocyst of a mouse model of polycystic ovarian syndromes.

Peyghambari Fatemeh F Amanpour Saeid S Fayazi Mehri M Haddadi Mahnaz M Muhammadnejad Samad S Muhammadnejad Ahad A Salimi Mehdi M Mazaheri Zohreh Z

Iranian journal of reproductive medicine 20140901 9

<h4>Background</h4>It has been hypothesized that blastocyst integrin expression changes can affect the spontaneous miscarriage in polycystic ovarian syndromes (PCOS).<h4>Objective</h4>In this study, the profile of integrin genes and proteins was investigated on blastocyst of the PCOS experimental mouse model.<h4>Materials and methods</h4>30 NMRI female mice were equally divided into 3 groups: control, experimental [PCOS that was injected estradiol valerate (40 mg/kg)]. After 8 weeks, each group ...[more]

PMID: 25469135

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Project description:IntroductionPolycystic ovarian syndrome (PCOS) is a common endocrine disorder amongst reproductive-age women, and 61% to 76% of women with PCOS are obese. Obese women with PCOS are usually burdened with infertility problems due to implantation failure. Thus, progesterone treatment is usually used to improve implantation rates. Although Hb-EGF expression is actively involved in endometrial receptivity and implantation, the data on heparin-binding epidermal growth factor (Hb-EGF) expression following progesterone therapy in obese women with PCOS are still lacking.ObjectiveTo investigate the changes in serum follicle-stimulating hormone (FSH), luteinising hormone (LH), dehydroepiandrosterone sulphate (DHEA), progesterone and oestradiol levels and Hb-EGF expression in obese women with PCOS during the implantation window following progesterone therapy.MethodA total of 40 participants aged 18-40 years old were recruited following the provision of written consent. The participants were divided into the obese PCOS, normal-weight PCOS, obese fertile and normal-weight fertile groups. First blood collection was done before ovulation. Then, daily oral micronised progesterone (Utrogestan 200 mg) was given to the PCOS group for 10 days. The treatment was followed by a second blood collection and endometrial tissue sampling by using a Pipelle de Cornier catheter. In the fertile group, ovulation was confirmed by using ultrasound, and a second blood sample was collected on days 7 to 9 postovulation. The serum levels of FSH, LH, DHEA, progesterone and oestradiol were measured in all participants. Wilcoxon signed-rank test was used to compare FSH, LH, DHEA, progesterone and oestradiol levels during pre- and postovulation. Mann-Whitney test was performed to compare FSH, LH, DHEA, progesterone and oestradiol levels between two groups: (1) the PCOS group and the fertile group, (2) the obese PCOS group and the non-obese PCOS group and (3) the obese group and the non-obese fertile group.ResultSerum FSH levels were lower in obese women in their follicular phase than in women with normal weight regardless of their PCOS status, whereas serum LH/FSH ratios and DHEA levels were higher in women with PCOS than in women without PCOS. However, endometrial Hb-EGF expression was lower in the obese PCOS group than in the normal-weight PCOS group.ConclusionsDifferent patterns of hormonal levels and Hb-EGF expression levels were seen between the studied groups. However, further in vitro and in vivo studies are needed to investigate the mechanism underlying the changes in FSH, LH/FSH ratio, DHEA and Hb-EGF expression in PCOS after progesterone treatment.

Dataset Information

Expression of ?4, ?v, ?1 and ?3 integrins during the implantation window on blastocyst of a mouse model of polycystic ovarian syndromes.

Publications

Expression of α4, αv, β1 and β3 integrins during the implantation window on blastocyst of a mouse model of polycystic ovarian syndromes.

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