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Assessment of ?4, ?v, ?1 and ?3 integrins expression throughout the implantation window phase in endometrium of a mouse model of polycystic ovarian syndromes.

ABSTRACT: BACKGROUND:Endometrial integrin expression changes might be a reason for implantation failure in polycystic ovarian syndromes (PCOS). Objective : Assessment of integrin genes and proteins expression upon endometrium in the PCOS experimental mouse model was the main goal of this study. MATERIALS AND METHODS:30 NMRI female mice were equally divided into control, experimental (PCOS; received estradiol valerate (40 mg/kg)) and sham group (received; olive oil). After 8 weeks, each group was hyper stimulated by 7 IU PMSG and then, after 48hrs, 7 IU HCG was injected. Vaginal plaque was checked. After 5 days, Progesterone and estradiol levels and endometrial tissues were investigated to evaluate of ?4, ?v, ?1 and ?3 integrins gene and protein by qPCR method and immunohistochemistry, respectively. RESULTS:Tissue samples were assessed and showed that level of progesterone was significantly decreased in PCOS group. RESULTS of molecular part in the amount of ?v, ?3, ?1 and ?4 gene expressions showed a great difference in ?3 and ?v genes expressions between experimental groups. ?v, ?3, ?4 and ?1 proteins in the endometrial stroma in the control group were expressed, but they were not detected in PCOS group. CONCLUSION:According to the results, integrins had different expression patterns in different areas of the endometrium; such as epithelial and stromal. It seems that in PCOS, this pattern has changed and the results might have a great influence on implantation failure. Therefore, this study suggests that a great attention to this problem may be essential in patients who are involved.

SUBMITTER: Peyghambari F

PROVIDER: S-EPMC4248155 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature

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Assessment of α4, αv, β1 and β3 integrins expression throughout the implantation window phase in endometrium of a mouse model of polycystic ovarian syndromes.

Peyghambari Fatemeh F Fayazi Mehri M Amanpour Saeid S Haddadi Mahnaz M Muhammadnejad Samad S Muhammadnejad Ahad A Abdolahpour Samira S Enkesari Mozhgan M Mazaheri Zohreh Z

Iranian journal of reproductive medicine 20141001 10

<h4>Background</h4>Endometrial integrin expression changes might be a reason for implantation failure in polycystic ovarian syndromes (PCOS). Objective : Assessment of integrin genes and proteins expression upon endometrium in the PCOS experimental mouse model was the main goal of this study.<h4>Materials and methods</h4>30 NMRI female mice were equally divided into control, experimental (PCOS; received estradiol valerate (40 mg/kg)) and sham group (received; olive oil). After 8 weeks, each grou ...[more]

PMID: 25469127

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Project description:BackgroundEndometriosis (EM) is highly associated with infertility. The precise mechanism underlying EM-associated infertility remains controversial. This study aimed to investigate the pathogenesis of infertility in women with EM by comparing FoxP3+ T regulatory cells (Tregs) expression in the eutopic endometrium of infertile women with EM and endometrium from healthy fertile women.MethodsAs a marker of Tregs, FoxP3 expression was analyzed in eutopic endometrium during the peri-implantation phase in infertile women with mild EM (n = 7), advanced EM (n = 20), and normally fertile women without EM (n = 20). FoxP3 mRNA expression was analyzed by quantitative real-time RT-PCR. FoxP3 protein expression was assessed by immunohistochemistry.ResultsFoxP3 mRNA expression in all infertile patients with EM was significantly higher than the control group (P < 0.05) by non-parametric Mann-Whitney U-test. Further analysis based on the extent of EM revealed that FoxP3 mRNA expression in infertile patients with advanced EM was significantly higher than the mild EM group and the control group (P < 0.05). Immunohistochemistry analysis showed predominant positive staining for FoxP3 protein in the endometrial stroma. In addition, the number of FoxP3+ cells in the eutopic endometrium of infertile women with advanced EM was marginally higher than the mild EM group and the control group, although the differences were not statistically significant (P > 0.05) by two-tailed t-tests.ConclusionsThese findings suggest that FoxP3+ Tregs in the peri-implantation endometrium might participate in the pathogenesis of advanced EM. However, they are not directly involved in the pathogenesis of advanced EM associated with infertility. The differential expression of FoxP3 in infertile women with mild EM and advanced EM implicates that notable differences in the uterine immune status are likely involved in the pathogenesis of mild EM associated with infertility in the peri-implantation endometrium.

Dataset Information

Assessment of ?4, ?v, ?1 and ?3 integrins expression throughout the implantation window phase in endometrium of a mouse model of polycystic ovarian syndromes.

Publications

Assessment of α4, αv, β1 and β3 integrins expression throughout the implantation window phase in endometrium of a mouse model of polycystic ovarian syndromes.

Similar Datasets

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