Ontology highlight
ABSTRACT:
SUBMITTER: Essletzbichler P
PROVIDER: S-EPMC4248322 | biostudies-literature | 2014 Dec
REPOSITORIES: biostudies-literature
Essletzbichler Patrick P Konopka Tomasz T Santoro Federica F Chen Doris D Gapp Bianca V BV Kralovics Robert R Brummelkamp Thijn R TR Nijman Sebastian M B SM Bürckstümmer Tilmann T
Genome research 20141104 12
Near-haploid human cell lines are instrumental for genetic screens and genome engineering as gene inactivation is greatly facilitated by the absence of a second gene copy. However, no completely haploid human cell line has been described, hampering the genetic accessibility of a subset of genes. The near-haploid human cell line HAP1 contains a single copy of all chromosomes except for a heterozygous 30-megabase fragment of Chromosome 15. This large fragment encompasses 330 genes and is integrate ...[more]