AFSM sequencing approach: a simple and rapid method for genome-wide SNP and methylation site discovery and genetic mapping.
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ABSTRACT:
SUBMITTER: Xia Z
PROVIDER: S-EPMC4252907 | biostudies-literature | 2014
REPOSITORIES: biostudies-literature
Publications
AFSM sequencing approach: a simple and rapid method for genome-wide SNP and methylation site discovery and genetic mapping.
Xia Zhiqiang Z Zou Meiling M Zhang Shengkui S Feng Binxiao B Wang Wenquan W
Scientific reports 20141203
We describe methods for the assessment of amplified-fragment single nucleotide polymorphism and methylation (AFSM) sites using a quick and simple molecular marker-assisted breeding strategy based on the use of two restriction enzyme pairs (EcoRI-MspI and EcoRI-HpaII) and a next-generation sequencing platform. Two sets of 85 adapter pairs were developed to concurrently identify SNPs, indels and methylation sites for 85 lines of cassava population in this study. In addition to SNPs and indels, the ...[more]
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