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An amphipathic polypeptide derived from poly-?-glutamic acid for the stabilization of membrane proteins.


ABSTRACT: Difficulties in the extraction of membrane proteins from cell membrane and their solubilization in native conformations have hindered their structural and biochemical analysis. To overcome these difficulties, an amphipathic polypeptide was synthesized by the conjugation of octyl and glucosyl groups to the carboxyl groups of poly-?-glutamic acid (PGA). This polymer, called amphipathic PGA (APG), self-assembles as mono-disperse oligomers consisted of 4-5 monomers. APG shows significantly low value of critical micelle concentration and stabilization activity toward membrane proteins. Most of the sodium dodecyl sulfate (SDS)-solubilized membrane proteins from Escherichia coli remain soluble state in the presence of APG even after the removal of SDS. In addition, APG stabilizes purified 7 transmembrane proteins such as bacteriorhodopsin and human endothelin receptor Type A (ETA ) in their active conformations. Furthermore, ETA in complex with APG is readily inserted into liposomes without disrupting the integrity of liposomes. These properties of APG can be applied to overcome the difficulties in the stabilization and reconstitution of membrane proteins.

SUBMITTER: Han SG 

PROVIDER: S-EPMC4253820 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature

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An amphipathic polypeptide derived from poly-γ-glutamic acid for the stabilization of membrane proteins.

Han Seong-Gu SG   Na Jung-Hyun JH   Lee Won-Kyu WK   Park Dongkook D   Oh Jihye J   Yoon Sung-Ho SH   Lee Cheng-Kang CK   Sung Moon-Hee MH   Shin Yeon-Kyun YK   Yu Yeon Gyu YG  

Protein science : a publication of the Protein Society 20141021 12


Difficulties in the extraction of membrane proteins from cell membrane and their solubilization in native conformations have hindered their structural and biochemical analysis. To overcome these difficulties, an amphipathic polypeptide was synthesized by the conjugation of octyl and glucosyl groups to the carboxyl groups of poly-γ-glutamic acid (PGA). This polymer, called amphipathic PGA (APG), self-assembles as mono-disperse oligomers consisted of 4-5 monomers. APG shows significantly low value  ...[more]

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