Ontology highlight
ABSTRACT:
SUBMITTER: Gilbert LA
PROVIDER: S-EPMC4253859 | biostudies-literature | 2014 Oct
REPOSITORIES: biostudies-literature
Gilbert Luke A LA Horlbeck Max A MA Adamson Britt B Villalta Jacqueline E JE Chen Yuwen Y Whitehead Evan H EH Guimaraes Carla C Panning Barbara B Ploegh Hidde L HL Bassik Michael C MC Qi Lei S LS Kampmann Martin M Weissman Jonathan S JS
Cell 20141009 3
While the catalog of mammalian transcripts and their expression levels in different cell types and disease states is rapidly expanding, our understanding of transcript function lags behind. We present a robust technology enabling systematic investigation of the cellular consequences of repressing or inducing individual transcripts. We identify rules for specific targeting of transcriptional repressors (CRISPRi), typically achieving 90%-99% knockdown with minimal off-target effects, and activator ...[more]