Combining TGF-? signal inhibition and connexin43 silencing for iPSC induction from mouse cardiomyocytes.
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ABSTRACT:
SUBMITTER: Dai P
PROVIDER: S-EPMC4255192 | biostudies-literature | 2014 Dec
REPOSITORIES: biostudies-literature
Publications
Combining TGF-β signal inhibition and connexin43 silencing for iPSC induction from mouse cardiomyocytes.
Dai Ping P Harada Yoshinori Y Miyachi Hitoshi H Tanaka Hideo H Kitano Satsuki S Adachi Tetsuya T Suzuki Tomoyuki T Hino Hitoshi H Takamatsu Tetsuro T
Scientific reports 20141204
The reprogramming of differentiated cells into induced pluripotent stem cells (iPSCs) can be achieved by ectopic expression of defined transcription factors (Oct3/4, Sox2, Klf4 and c-Myc). However, to date, some iPSCs have been generated using viral vectors; thus, unexpected insertional mutagenesis in the target cells would be a potential risk. Here we report reprogramming of siPSCs (gene silencing-induced pluripotent stem cells) from mouse neonatal cardiomyocytes (CMs) by combining TGF-β signal ...[more]
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