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Two-dimensionality of yeast colony expansion accompanied by pattern formation.


ABSTRACT: Yeasts can form multicellular patterns as they expand on agar plates, a phenotype that requires a functional copy of the FLO11 gene. Although the biochemical and molecular requirements for such patterns have been examined, the mechanisms underlying their formation are not entirely clear. Here we develop quantitative methods to accurately characterize the size, shape, and surface patterns of yeast colonies for various combinations of agar and sugar concentrations. We combine these measurements with mathematical and physical models and find that FLO11 gene constrains cells to grow near the agar surface, causing the formation of larger and more irregular colonies that undergo hierarchical wrinkling. Head-to-head competition assays on agar plates indicate that two-dimensional constraint on the expansion of FLO11 wild type (FLO11) cells confers a fitness advantage over FLO11 knockout (flo11?) cells on the agar surface.

SUBMITTER: Chen L 

PROVIDER: S-EPMC4263361 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature

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Two-dimensionality of yeast colony expansion accompanied by pattern formation.

Chen Lin L   Noorbakhsh Javad J   Adams Rhys M RM   Samaniego-Evans Joseph J   Agollah Germaine G   Nevozhay Dmitry D   Kuzdzal-Fick Jennie J   Mehta Pankaj P   Balázsi Gábor G  

PLoS computational biology 20141211 12


Yeasts can form multicellular patterns as they expand on agar plates, a phenotype that requires a functional copy of the FLO11 gene. Although the biochemical and molecular requirements for such patterns have been examined, the mechanisms underlying their formation are not entirely clear. Here we develop quantitative methods to accurately characterize the size, shape, and surface patterns of yeast colonies for various combinations of agar and sugar concentrations. We combine these measurements wi  ...[more]

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