Ontology highlight
ABSTRACT: Background
Dendritic cells are currently under investigation for their ability to generate anti-cancer immune responses. No consensus has been reached as to the optimal method of dendritic cell vaccine preparation and is a barrier to success in the field.Methods
Over a course of three separate dendritic cell vaccine studies to treat cancer, we tested two different methods for preparing dendritic cells from peripheral blood mononuclear cells: adherence and antibody-selected CD14+ cells.Results
Surprisingly, we found that patients who received dendritic cell vaccines generated by the adherence method mounted increased T cell proliferation in response to vaccination. This difference could not be accounted for by dendritic cell vaccine dose, cell surface phenotype or dendritic cell function in vitro. One notable difference between the two vaccine preparation methods was that the dendritic cell vaccine cultures generated by the adherence method contained up to 10% lymphocytes, and these lymphocytes were proliferating and producing IFNγ in response to antigen in vitro at the time of administration.Conclusions
Enhanced immunogenicity of adherence dendritic cell vaccinations may be due to the presence of lymphocytes during dendritic cell culture.Trial registration
Clinicaltrials.gov identifiers: NCT00289341, NCT00345293, and NCT00893945.
SUBMITTER: Frank MO
PROVIDER: S-EPMC4264264 | biostudies-literature | 2014 Dec
REPOSITORIES: biostudies-literature
Frank Mayu O MO Kaufman Julia J Parveen Salina S Blachère Nathalie E NE Orange Dana E DE Darnell Robert B RB
Journal of translational medicine 20141205
<h4>Background</h4>Dendritic cells are currently under investigation for their ability to generate anti-cancer immune responses. No consensus has been reached as to the optimal method of dendritic cell vaccine preparation and is a barrier to success in the field.<h4>Methods</h4>Over a course of three separate dendritic cell vaccine studies to treat cancer, we tested two different methods for preparing dendritic cells from peripheral blood mononuclear cells: adherence and antibody-selected CD14+ ...[more]