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Characterization of polyploid wheat genomic diversity using a high-density 90,000 single nucleotide polymorphism array.


ABSTRACT: High-density single nucleotide polymorphism (SNP) genotyping arrays are a powerful tool for studying genomic patterns of diversity, inferring ancestral relationships between individuals in populations and studying marker-trait associations in mapping experiments. We developed a genotyping array including about 90,000 gene-associated SNPs and used it to characterize genetic variation in allohexaploid and allotetraploid wheat populations. The array includes a significant fraction of common genome-wide distributed SNPs that are represented in populations of diverse geographical origin. We used density-based spatial clustering algorithms to enable high-throughput genotype calling in complex data sets obtained for polyploid wheat. We show that these model-free clustering algorithms provide accurate genotype calling in the presence of multiple clusters including clusters with low signal intensity resulting from significant sequence divergence at the target SNP site or gene deletions. Assays that detect low-intensity clusters can provide insight into the distribution of presence-absence variation (PAV) in wheat populations. A total of 46 977 SNPs from the wheat 90K array were genetically mapped using a combination of eight mapping populations. The developed array and cluster identification algorithms provide an opportunity to infer detailed haplotype structure in polyploid wheat and will serve as an invaluable resource for diversity studies and investigating the genetic basis of trait variation in wheat.

SUBMITTER: Wang S 

PROVIDER: S-EPMC4265271 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature

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Characterization of polyploid wheat genomic diversity using a high-density 90,000 single nucleotide polymorphism array.

Wang Shichen S   Wong Debbie D   Forrest Kerrie K   Allen Alexandra A   Chao Shiaoman S   Huang Bevan E BE   Maccaferri Marco M   Salvi Silvio S   Milner Sara G SG   Cattivelli Luigi L   Mastrangelo Anna M AM   Whan Alex A   Stephen Stuart S   Barker Gary G   Wieseke Ralf R   Plieske Joerg J   Lillemo Morten M   Mather Diane D   Appels Rudi R   Dolferus Rudy R   Brown-Guedira Gina G   Korol Abraham A   Akhunova Alina R AR   Feuillet Catherine C   Salse Jerome J   Morgante Michele M   Pozniak Curtis C   Luo Ming-Cheng MC   Dvorak Jan J   Morell Matthew M   Dubcovsky Jorge J   Ganal Martin M   Tuberosa Roberto R   Lawley Cindy C   Mikoulitch Ivan I   Cavanagh Colin C   Edwards Keith J KJ   Hayden Matthew M   Akhunov Eduard E  

Plant biotechnology journal 20140320 6


High-density single nucleotide polymorphism (SNP) genotyping arrays are a powerful tool for studying genomic patterns of diversity, inferring ancestral relationships between individuals in populations and studying marker-trait associations in mapping experiments. We developed a genotyping array including about 90,000 gene-associated SNPs and used it to characterize genetic variation in allohexaploid and allotetraploid wheat populations. The array includes a significant fraction of common genome-  ...[more]

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