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Granulomatous response to Coxiella burnetii, the agent of Q fever: the lessons from gene expression analysis.


ABSTRACT: The formation of granulomas is associated with the resolution of Q fever, a zoonosis due to Coxiella burnetii; however the molecular mechanisms of granuloma formation remain poorly understood. We generated human granulomas with peripheral blood mononuclear cells (PBMCs) and beads coated with C. burnetii, using BCG extracts as controls. A microarray analysis showed dramatic changes in gene expression in granuloma cells of which more than 50% were commonly modulated genes in response to C. burnetii and BCG. They included M1-related genes and genes related to chemotaxis. The inhibition of the chemokines, CCL2 and CCL5, directly interfered with granuloma formation. C. burnetii granulomas also expressed a specific transcriptional profile that was essentially enriched in genes associated with type I interferon response. Our results showed that granuloma formation is associated with a core of transcriptional response based on inflammatory genes. The specific granulomatous response to C. burnetii is characterized by the activation of type 1 interferon pathway.

SUBMITTER: Faugaret D 

PROVIDER: S-EPMC4266094 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature

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Granulomatous response to Coxiella burnetii, the agent of Q fever: the lessons from gene expression analysis.

Faugaret Delphine D   Ben Amara Amira A   Alingrin Julie J   Daumas Aurélie A   Delaby Amélie A   Lépolard Catherine C   Raoult Didier D   Textoris Julien J   Mège Jean-Louis JL  

Frontiers in cellular and infection microbiology 20141215


The formation of granulomas is associated with the resolution of Q fever, a zoonosis due to Coxiella burnetii; however the molecular mechanisms of granuloma formation remain poorly understood. We generated human granulomas with peripheral blood mononuclear cells (PBMCs) and beads coated with C. burnetii, using BCG extracts as controls. A microarray analysis showed dramatic changes in gene expression in granuloma cells of which more than 50% were commonly modulated genes in response to C. burneti  ...[more]

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