Identification of the WNT1 residues required for palmitoylation by Porcupine.
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ABSTRACT:
SUBMITTER: Miranda M
PROVIDER: S-EPMC4268045 | biostudies-literature | 2014 Dec
REPOSITORIES: biostudies-literature
Publications
Identification of the WNT1 residues required for palmitoylation by Porcupine.
Miranda M M Galli L M LM Enriquez M M Szabo L A LA Gao X X Hannoush R N RN Burrus L W LW
FEBS letters 20141120 24
The post-translational palmitoylation of WNT morphogens is critical for proper signaling during embryogenesis and adult homeostasis. The addition of palmitoyl groups to WNT proteins is catalyzed by Porcupine (PORCN). However, the Wnt amino acid residues required for recognition and palmitoylation by PORCN have not been fully characterized. We show that WNT1 residues 214-234 are sufficient for PORCN-dependent palmitoylation of Ser224. Substitution of Ser224 with Thr, but not Cys, is tolerated in ...[more]
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