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Residence times of receptors in dendritic spines analyzed by stochastic simulations in empirical domains.

ABSTRACT: Analysis of high-density superresolution imaging of receptors reveals the organization of dendrites at nanoscale resolution. We present here an apparently novel method that uses local statistics extracted from short-range trajectories for the simulations of long-range trajectories in empirical live cell images. Based on these empirical simulations, we compute the residence time of a receptor in dendritic spines that accounts for receptors' local interactions and geometrical membrane organization. We report here that depending on the type of the spine, the residence time varies from 1 to 5 min. Moreover, we show that there exists transient organized structures, previously described as potential wells that can regulate the trafficking of receptors to dendritic spine: the simulation results suggest that receptor trafficking is regulated by transient structures.

SUBMITTER: Hoze N

PROVIDER: S-EPMC4269777 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature

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Residence times of receptors in dendritic spines analyzed by stochastic simulations in empirical domains.

Hoze Nathanael N Holcman David D

Biophysical journal 20141201 12

Analysis of high-density superresolution imaging of receptors reveals the organization of dendrites at nanoscale resolution. We present here an apparently novel method that uses local statistics extracted from short-range trajectories for the simulations of long-range trajectories in empirical live cell images. Based on these empirical simulations, we compute the residence time of a receptor in dendritic spines that accounts for receptors' local interactions and geometrical membrane organization ...[more]

PMID: 25517165

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