Mouse dead end 1-? interacts with c-Jun and stimulates activator protein 1 transactivation.

ABSTRACT: Dead end 1 (DND1), important for maintaining the viability of primordial germ cells, is the first protein containing an RNA recognition motif that has been directly implicated as a heritable cause of spontaneous tumorigenesis. In the present study, c-Jun was identified through yeast two-hybrid screening of a 10.5-day old mouse embryo cDNA library as one of the proteins which interact with DND1-?. The interaction between DND1-? and c-Jun was demonstrated to occur by glutathione S?transferase pull?down and co-immunoprecipitation. Using confocal microscopy, DND1-? was found to be specifically expressed in GC-1 spermatogonia cells, mainly in the nuclei. When transfected into GC-1 cells, DND1-? and c-Jun were demonstrated to be co-localized principally in the nuclei. Furthermore, in a dual luciferase reporter assay, the transcriptional activity of activator protein 1 was demonstrated to be significantly increased by co-transfection with DND1-? and c-Jun plasmids in GC-1 cells. The identification and confirmation of an additional protein interacting with DND1-? facilitates the investigation of the functions and molecular mechanisms of DND1.

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC4270339 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature