Ontology highlight
ABSTRACT:
SUBMITTER: Knorr RL
PROVIDER: S-EPMC4270758 | biostudies-literature | 2014
REPOSITORIES: biostudies-literature
Knorr Roland L RL Nakatogawa Hitoshi H Ohsumi Yoshinori Y Lipowsky Reinhard R Baumgart Tobias T Dimova Rumiana R
PloS one 20141218 12
Autophagy is a cellular degradation pathway involving the shape transformation of lipid bilayers. During the onset of autophagy, the water-soluble protein Atg8 binds covalently to phosphatdylethanolamines (PEs) in the membrane in an ubiquitin-like reaction coupled to ATP hydrolysis. We reconstituted the Atg8 conjugation system in giant and nm-sized vesicles with a minimal set of enzymes and observed that formation of Atg8-PE on giant vesicles can cause substantial tubulation of membranes even in ...[more]