Human V?2(+) ?? T Cells Differentially Induce Maturation, Cytokine Production, and Alloreactive T Cell Stimulation by Dendritic Cells and B Cells.

ABSTRACT: Human ?? T cells expressing the V?9V?2 T cell receptor can induce maturation of dendritic cells (DC) into antigen-presenting cells (APC) and B cells into antibody-secreting plasma cells. Since B cells are capable of presenting antigens to T cells, we investigated if V?9V?2 T cells can influence antigen-presentation by these cells. We report that V?9V?2 T cells induced expression of CD86, HLA-DR, and CD40 by B cells and stimulated the release of IL-4, IL-6, TNF-?, and IgG, IgA, and IgM. V?9V?2 T cells also augmented the ability of B cells to stimulate proliferation but not IFN-? or IL-4 release by alloreactive T cells. In contrast, V?9V?2 T cells induced expression of CD86 and HLA-DR and the release of IFN-?, IL-6, and TNF-? by DC and these DC stimulated proliferation and IFN-? production by conventional T cells. Furthermore, CD86, TNF-?, IFN-?, and cell contact were found to be important in DC activation by V?9V?2 T cells but not in the activation of B cells. These data suggest that V?9V?2 T cells can induce maturation of B cells and DC into APC, but while they prime DC to stimulate T helper 1 (TH1) responses, they drive maturation of B cells into APC that can stimulate different T cell responses. Thus, V?9V?2 T cells can control different arms of the immune system through selective activation of B cells and DC in vitro, which may have important applications in immunotherapy and for vaccine adjuvants.

SUBMITTER: Petrasca A 

PROVIDER: S-EPMC4271703 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature