Dataset Information

Plasma microRNAs are associated with atrial fibrillation and change after catheter ablation (the miRhythm study).

ABSTRACT: MicroRNAs (miRNAs) are associated with cardiovascular disease and control gene expression and are detectable in the circulation.The purpose of this study was to test the hypothesis that circulating miRNAs may be associated with atrial fibrillation (AF).Using a prospective study design powered to detect subtle differences in miRNAs, we quantified plasma expression of 86 miRNAs by high-throughput quantitative reverse transcriptase-polymerase chain reaction in 112 participants with AF and 99 without AF. To examine parallels between cardiac and plasma miRNA profiles, we quantified atrial tissue and plasma miRNA expression using quantitative reverse transcriptase-polymerase chain reaction in 31 participants undergoing surgery. We also explored the hypothesis that lower AF burden after ablation would be reflected in the circulating blood pool by examining change in plasma miRNAs after AF ablation (n = 47).Mean age of the cohort was 59 years; 58% of participants were men. Plasma miRs-21 and 150 were 2-fold lower in participants with AF than in those without AF after adjustment (P ?.0006). Plasma levels of miRs-21 and 150 also were lower in participants with paroxysmal AF than in those with persistent AF (P <.05). Expression of miR-21, but not of miR-150, was lower in atrial tissue from patients with AF than in those without AF (P <.05). Plasma levels of miRs-21 and 150 increased 3-fold after AF ablation (P ?.0006).Cardiac miRs-21 and 150 are known to regulate genes implicated in atrial remodeling. Our findings show associations between plasma miRs-21 and 150 and AF, suggesting that circulating miRNAs can provide insights into cardiac gene regulation.

SUBMITTER: McManus DD

PROVIDER: S-EPMC4277933 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature

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Plasma microRNAs are associated with atrial fibrillation and change after catheter ablation (the miRhythm study).

McManus David D DD Tanriverdi Kahraman K Lin Honghuang H Esa Nada N Kinno Menhel M Mandapati Divakar D Tam Stanley S Okike Okike N ON Ellinor Patrick T PT Keaney John F JF Donahue J Kevin JK Benjamin Emelia J EJ Freedman Jane E JE

Heart rhythm 20141009 1

<h4>Background</h4>MicroRNAs (miRNAs) are associated with cardiovascular disease and control gene expression and are detectable in the circulation.<h4>Objective</h4>The purpose of this study was to test the hypothesis that circulating miRNAs may be associated with atrial fibrillation (AF).<h4>Methods</h4>Using a prospective study design powered to detect subtle differences in miRNAs, we quantified plasma expression of 86 miRNAs by high-throughput quantitative reverse transcriptase-polymerase cha ...[more]

PMID: 25257092

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Project description:AF is a heterogeneous rhythm disorder that is related to a wide spectrum of etiologies and has broad clinical presentations. Mechanisms underlying AF are complex and remain incompletely understood despite extensive research. They associate interactions between triggers, substrate and modulators including ionic and anatomic remodeling, genetic predisposition and neuro-humoral contributors. The pulmonary veins play a key role in the pathogenesis of AF and their isolation is associated to high rates of AF freedom in patients with paroxysmal AF. However, ablation of persistent AF remains less effective, mainly limited by the difficulty to identify the sources sustaining AF. Many theories were advanced to explain the perpetuation of this form of AF, ranging from a single localized focal and reentrant source to diffuse bi-atrial multiple wavelets. Translating these mechanisms to the clinical practice remains challenging and limited by the spatio-temporal resolution of the mapping techniques. AF is driven by focal or reentrant activities that are initially clustered in a relatively limited atrial surface then disseminate everywhere in both atria. Evidence for structural remodeling, mainly represented by atrial fibrosis suggests that reentrant activities using anatomical substrate are the key mechanism sustaining AF. These reentries can be endocardial, epicardial, and intramural which makes them less accessible for mapping and for ablation. Subsequently, early interventions before irreversible remodeling are of major importance. Circumferential pulmonary vein isolation remains the cornerstone of the treatment of AF, regardless of the AF form and of the AF duration. No ablation strategy consistently demonstrated superiority to pulmonary vein isolation in preventing long term recurrences of atrial arrhythmias. Further research that allows accurate identification of the mechanisms underlying AF and efficient ablation should improve the results of PsAF ablation.

Dataset Information

Plasma microRNAs are associated with atrial fibrillation and change after catheter ablation (the miRhythm study).

Publications

Plasma microRNAs are associated with atrial fibrillation and change after catheter ablation (the miRhythm study).

Similar Datasets

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