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Mechanisms Underlying the Antinociceptive, Antiedematogenic, and Anti-Inflammatory Activity of the Main Flavonoid from Kalanchoe pinnata.

ABSTRACT: Kalanchoe pinnata (KP) is popularly used for treating inflammatory diseases. This study investigated the antinociceptive, antiedematogenic, and anti-inflammatory potential of the subcutaneous administration of KP flower aqueous extract (KPFE), its ethyl acetate (EtOAcF) and butanol (BuOHF) fractions, and the main KP flavonoid [quercetin 3-O-?-L-arabinopyranosyl (1 ? 2) ?-L-rhamnopyranoside] (KPFV) in mice, as well as its possible mechanisms of action. KPFE (30-300?mg/kg) and KPFV (1-10?mg/kg) inhibited the acetic acid-induced writhing (ID50 = 164.8 and 9.4?mg/kg, resp.). KPFE (300?mg/kg), EtOAcF (12?mg/kg), BuOHF (15?mg/kg), or KPFV (0.3-3.0?mg/kg) reduced leukocyte migration on carrageenan-induced pleurisy (ID50 = 2.0?mg/kg for KPFV). KPFE (3-30?mg/kg) and KPFV (0.3-3.0?mg/kg) reduced the croton oil-induced ear edema (ID50 = 4.3 and 0.76?mg/kg, resp.). KPFE and KPFV reduced the TNF-? concentration in the pleural exudates on carrageenan-induced pleurisy test. Moreover, KPFV inhibited COX-1 (IC50 = 22.1??g/mL) and COX-2 (IC50 > 50??g/mL). The selectivity index (COX-1IC50 /COX-2IC50 ) was <0.44. These results indicate that KPFE and KPFV produced antinociceptive, antiedematogenic, and anti-inflammatory activities through COX inhibition and TNF-? reduction, revealing that the main flavonoid in KP flowers and leaves plays an important role in the ethnomedicinal use of the plant.

SUBMITTER: Ferreira RT

PROVIDER: S-EPMC4279175 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature

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Mechanisms Underlying the Antinociceptive, Antiedematogenic, and Anti-Inflammatory Activity of the Main Flavonoid from Kalanchoe pinnata.

Ferreira Raquel Teixeira RT Coutinho Marcela Araújo Soares MA Malvar David do Carmo Ddo C Costa Elson Alves EA Florentino Iziara Ferreira IF Costa Sônia Soares SS Vanderlinde Frederico Argollo FA

Evidence-based complementary and alternative medicine : eCAM 20141211

Kalanchoe pinnata (KP) is popularly used for treating inflammatory diseases. This study investigated the antinociceptive, antiedematogenic, and anti-inflammatory potential of the subcutaneous administration of KP flower aqueous extract (KPFE), its ethyl acetate (EtOAcF) and butanol (BuOHF) fractions, and the main KP flavonoid [quercetin 3-O-α-L-arabinopyranosyl (1 → 2) α-L-rhamnopyranoside] (KPFV) in mice, as well as its possible mechanisms of action. KPFE (30-300 mg/kg) and KPFV (1-10 mg/kg) in ...[more]

PMID: 25580151

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Project description:The species Kalanchoe brasiliensis and Kalanchoe pinnata, both known popularly as "Saião," are used interchangeably in traditional medicine for their antiophidic properties. Studies evaluating the anti-venom activity of these species are scarce. This study aims to characterize the chemical constituents and evaluate the inhibitory effects of hydroethanolic leaf extracts of K. brasiliensis and K. pinnata against local effects induced by Bothrops jararaca snake venom. Thin Layer Chromatography (TLC) and High Performance Liquid Chromatography coupled with Diode Array Detection and Electrospray Mass Spectrometry (HPLC-DAD-MS/MS) were performed for characterization of chemical markers of the extracts from these species. For antiophidic activity evaluation, B. jararaca venom-induced paw edema and skin hemorrhage in mice were evaluated. In both models, hydroethanolic extracts (125-500 mg/kg) were administered intraperitoneally in different protocols. Inhibition of phospholipase enzymatic activity of B. jararaca was evaluated. The HPLC-DAD-MS/MS chromatographic profile of extracts showed some particularities in the chemical profile of the two species. K. brasileinsis exhibited major peaks that have UV spectra similar to flavonoid glycosides derived from patuletin and eupafolin, while K. pinnata showed UV spectra similar to flavonoids glycosides derived from quercetin and kaempferol. Both extracts significantly reduced the hemorrhagic activity of B. jararaca venom in pre-treatment protocol, reaching about 40% of inhibition, while only K. pinnata was active in post-treatment protocol (about 30% of inhibition). In the antiedematogenic activity, only K. pinnata was active, inhibiting about 66% and 30% in pre and post-treatment protocols, respectively. Both extracts inhibited phospholipase activity; however, K. pinnata was more active. In conclusion, the results indicate the potential antiophidic activity of Kalanchoe species against local effects induced by B. jararaca snake venom, suggesting their potential use as a new source of bioactive molecules against bothropic venom.

Dataset Information

Mechanisms Underlying the Antinociceptive, Antiedematogenic, and Anti-Inflammatory Activity of the Main Flavonoid from Kalanchoe pinnata.

Publications

Mechanisms Underlying the Antinociceptive, Antiedematogenic, and Anti-Inflammatory Activity of the Main Flavonoid from Kalanchoe pinnata.

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