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Let-65 is cytoplasmic methionyl tRNA synthetase in C. elegans.

ABSTRACT: Cytoplasmic methionyl tRNA synthetase (MetRS) is one of more than 20 cytoplasmic aminoacyl tRNA synthetase enzymes (ARS). This family of enzymes catalyzes a process fundamental for protein translation. Using a combination of genetic mapping, oligonucleotide array comparative genomic hybridization, and phenotypic correlation, we show that mutations in the essential gene, let-65, reside within the predicted Caenorhabditis elegans homologue of MetRS, which we have named mars-1. We demonstrate that the lethality associated with alleles of let-65 is fully rescued by a transgenic array that spans the mars-1 genomic region. Furthermore, sequence analysis reveals that six let-65 alleles lead to the alteration of highly conserved amino acids.

SUBMITTER: Alriyami MZ

PROVIDER: S-EPMC4287814 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature

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let-65 is cytoplasmic methionyl tRNA synthetase in C. elegans.

Alriyami Maha Z MZ Jones Martin R MR Johnsen Robert C RC Banerjee Yajnavalka Y Baillie David L DL

Meta gene 20141109

Cytoplasmic methionyl tRNA synthetase (MetRS) is one of more than 20 cytoplasmic aminoacyl tRNA synthetase enzymes (ARS). This family of enzymes catalyzes a process fundamental for protein translation. Using a combination of genetic mapping, oligonucleotide array comparative genomic hybridization, and phenotypic correlation, we show that mutations in the essential gene, let-65, reside within the predicted Caenorhabditis elegans homologue of MetRS, which we have named mars-1. We demonstrate that ...[more]

PMID: 25606464

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