Ontology highlight
ABSTRACT:
SUBMITTER: Rath D
PROVIDER: S-EPMC4288158 | biostudies-literature | 2015 Jan
REPOSITORIES: biostudies-literature
Rath Devashish D Amlinger Lina L Hoekzema Mirthe M Devulapally Praneeth Reddy PR Lundgren Magnus M
Nucleic acids research 20141130 1
Methods that permit controlled changes in the expression of genes are important tools for biological and medical research, and for biotechnological applications. Conventional methods are directed at individually changing each gene, its regulatory elements or its mRNA's translation rate. We demonstrate that the CRISPR-associated DNA-binding Cascade complex can be used for efficient, long-lasting and programmable gene silencing. When Cascade is targeted to a promoter sequence the transcription of ...[more]