Dataset Information

Efficient Virus-Induced Gene Silencing in Solanum rostratum.

ABSTRACT: Solanum rostratum is a "super weed" that grows fast, is widespread, and produces the toxin solanine, which is harmful to both humans and other animals. To our knowledge, no study has focused on its molecular biology owing to the lack of available transgenic methods and sequence information for S. rostratum. Virus-induced gene silencing (VIGS) is a powerful tool for the study of gene function in plants; therefore, in the present study, we aimed to establish tobacco rattle virus (TRV)-derived VIGS in S. rostratum. The genes for phytoene desaturase (PDS) and Chlorophyll H subunit (ChlH) of magnesium protoporphyrin chelatase were cloned from S. rostratum and used as reporters of gene silencing. It was shown that high-efficiency VIGS can be achieved in the leaves, flowers, and fruit of S. rostratum. Moreover, based on our comparison of three different types of infection methods, true leaf infection was found to be more efficient than cotyledon and sprout infiltration in long-term VIGS in multiple plant organs. In conclusion, the VIGS technology and tomato genomic sequences can be used in the future to study gene function in S. rostratum.

SUBMITTER: Meng LH

PROVIDER: S-EPMC4892644 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature

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Efficient Virus-Induced Gene Silencing in Solanum rostratum.

Meng Lan-Huan LH Wang Rui-Heng RH Zhu Ben-Zhong BZ Zhu Hong-Liang HL Luo Yun-Bo YB Fu Da-Qi DQ

PloS one 20160603 6

Solanum rostratum is a "super weed" that grows fast, is widespread, and produces the toxin solanine, which is harmful to both humans and other animals. To our knowledge, no study has focused on its molecular biology owing to the lack of available transgenic methods and sequence information for S. rostratum. Virus-induced gene silencing (VIGS) is a powerful tool for the study of gene function in plants; therefore, in the present study, we aimed to establish tobacco rattle virus (TRV)-derived VIGS ...[more]

PMID: 27258320

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Project description:Background and aimsEnantiostyly is a reproductive system with heteromorphic flowers characterized by asymmetrical deflection of the style, either to the left or to the right of the floral axis. There are two types of enantiostyly. In monomorphic enantiostyly, plants produce the two types of flowers in the same individual. Dimorphic enantiostyly is restricted to only seven species and their populations consist of individuals producing either the right or the left flower type. It is hypothesized that the dimorphic form is derived from monomorphic ancestors because it functions as an outcrossing mechanism. We tested this latter hypothesis and investigated if monomorphic enantiostyly is resistant to invasion by individuals with dimorphic enantiostyly, because it functions as a reproductive assurance mechanism.MethodsTo determine the conditions favouring the invasion of dimorphic enantiostyly, measurements of reproductive success and outcrossing rates in 15 natural flowering patches of Solanum rostratum were made. To test if monomorphic enantiostyly provides a reproductive assurance mechanism, experimental plants with either manually created dimorphic or natural monomorphic reproductive systems were exposed to two different pollination scenarios (flower density treatments), and reproductive success and outcrossing rates were measured.Key resultsNaturally flowering patches experienced severe pollination limitation, showed marked differences in reproductive success and had relatively high outcrossing rates. Plants in the experimental patches also showed pollination limitation and high outcrossing rates. Individuals with dimorphic enantiostyly expressed higher reproductive and outcrossing advantages under high-density conditions. These advantages disappeared in the low-density treatment, where the monomorphic form attained a higher reproductive success and no differences in outcrossing rates were detected.ConclusionsMonomorphic enantiostyly should be resistant to invasion of the dimorphic form because the prevalent ecological conditions favour the maintenance of geitonogamous individuals that are able to take advantage of ecological heterogeneity and generalized pollination limitation.

Dataset Information

Efficient Virus-Induced Gene Silencing in Solanum rostratum.

Publications

Efficient Virus-Induced Gene Silencing in Solanum rostratum.

Similar Datasets

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