Ontology highlight
ABSTRACT:
SUBMITTER: Cheng HM
PROVIDER: S-EPMC4288177 | biostudies-literature | 2015 Jan
REPOSITORIES: biostudies-literature
Cheng Hsin-Mei HM Gröger Philip P Hartmann Andreas A Schlierf Michael M
Nucleic acids research 20141203 1
DNA replication initiation is mediated across all domains of life by initiator proteins oligomerizing at replication origins. Recently, it was shown that initiators can directly bind single-stranded DNA (ssDNA) and thus might enhance origin melting. In this study, we used single-molecule fluorescence assays to probe the ssDNA binding mechanism of the replication initiator DnaA. Our experiments revealed that DnaA forms a dynamic filament on ssDNA in 3' to 5' directionality in the presence of ATP ...[more]