Ontology highlight
ABSTRACT:
SUBMITTER: Carpy A
PROVIDER: S-EPMC4288259 | biostudies-literature | 2015 Jan
REPOSITORIES: biostudies-literature
Carpy Alejandro A Patel Avinash A Tay Ye Dee YD Hagan Iain M IM Macek Boris B
Molecular & cellular proteomics : MCP 20141103 1
Stable Isotope Labeling by Amino Acids (SILAC) is a commonly used method in quantitative proteomics. Because of compatibility with trypsin digestion, arginine and lysine are the most widely used amino acids for SILAC labeling. We observed that Schizosaccharomyces pombe (fission yeast) cannot be labeled with a specific form of arginine, (13)C(6) (15)N(4)-arginine (Arg-10), which limits the exploitation of SILAC technology in this model organism. We hypothesized that in the fission yeast the guani ...[more]