Ontology highlight
ABSTRACT:
SUBMITTER: Watanabe S
PROVIDER: S-EPMC4291189 | biostudies-literature | 2014 Nov
REPOSITORIES: biostudies-literature
Watanabe Shigeki S Trimbuch Thorsten T Camacho-Pérez Marcial M Rost Benjamin R BR Brokowski Bettina B Söhl-Kielczynski Berit B Felies Annegret A Davis M Wayne MW Rosenmund Christian C Jorgensen Erik M EM
Nature 20141008 7526
Ultrafast endocytosis can retrieve a single, large endocytic vesicle as fast as 50-100 ms after synaptic vesicle fusion. However, the fate of the large endocytic vesicles is not known. Here we demonstrate that these vesicles transition to a synaptic endosome about one second after stimulation. The endosome is resolved into coated vesicles after 3 s, which in turn become small-diameter synaptic vesicles 5-6 s after stimulation. We disrupted clathrin function using RNA interference (RNAi) and foun ...[more]