Ontology highlight
ABSTRACT:
SUBMITTER: Borner GH
PROVIDER: S-EPMC2064594 | biostudies-literature | 2006 Nov
REPOSITORIES: biostudies-literature
Borner Georg H H GH Harbour Michael M Hester Svenja S Lilley Kathryn S KS Robinson Margaret S MS
The Journal of cell biology 20061101 4
Clathrin-coated vesicles (CCVs) facilitate the transport of cargo between the trans-Golgi network, endosomes, and the plasma membrane. This study presents the first comparative proteomics investigation of CCVs. A CCV-enriched fraction was isolated from HeLa cells and a "mock CCV" fraction from clathrin-depleted cells. We used a combination of 2D difference gel electrophoresis and isobaric tags for relative and absolute quantification (iTRAQ) in conjunction with mass spectrometry to analyze and c ...[more]