Ontology highlight
ABSTRACT:
SUBMITTER: Curran TG
PROVIDER: S-EPMC4293043 | biostudies-literature | 2015 Jan
REPOSITORIES: biostudies-literature
Curran Timothy G TG Zhang Yi Y Ma Daniel J DJ Sarkaria Jann N JN White Forest M FM
Nature communications 20150112
Absolute quantification of protein expression and posttranslational modifications by mass spectrometry has been challenging due to a variety of factors, including the potentially large dynamic range of phosphorylation response. To address these issues, we have developed MARQUIS-Multiplex Absolute Regressed Quantification with Internal Standards-a novel mass spectrometry-based approach using a combination of isobaric tags and heavy-labelled standard peptides, to construct internal standard curves ...[more]