Ontology highlight
ABSTRACT:
SUBMITTER: Becker AK
PROVIDER: S-EPMC4294422 | biostudies-literature | 2015 Mar
REPOSITORIES: biostudies-literature
Becker Ann-Kathrin A AK Mikolajek Halina H Werner Jörn M JM Paulsson Mats M Wagener Raimund R
Protein expression and purification 20141121
VWA domains are the predominant independent folding units within matrilins and mediate protein-protein interactions. Mutations in the matrilin-3 VWA domain cause various skeletal diseases. The analysis of the pathological mechanisms is hampered by the lack of detailed structural information on matrilin VWA domains. Attempts to resolve their structures were hindered by low solubility and a tendency to aggregation. We therefore took a comprehensive approach to improve the recombinant expression of ...[more]