Use of gnotobiotic zebrafish to study Vibrio anguillarum pathogenicity.
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ABSTRACT:
SUBMITTER: Oyarbide U
PROVIDER: S-EPMC4298153 | biostudies-literature | 2015 Feb
REPOSITORIES: biostudies-literature
Publications
Use of gnotobiotic zebrafish to study Vibrio anguillarum pathogenicity.
Oyarbide Usua U Iturria Iñaki I Rainieri Sandra S Pardo Miguel Angel MA
Zebrafish 20141230 1
We evaluated the use of the gnotobiotic zebrafish system to study the effects of bacterial infection, and analyzed expression of genes involved in zebrafish innate immunity. Using a GFP-labeled strain of Vibrio anguillarum, we fluorescently monitored colonization of the zebrafish intestinal tract and used gene expression analysis to compare changes in genes involved in innate immunity between nongnotobiotic and gnotobiotic larvae. The experiments performed with the gnotobiotic zebrafish reveal n ...[more]
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