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Accurate quantification of episomal HIV-1 two-long terminal repeat circles by use of optimized DNA isolation and droplet digital PCR.

ABSTRACT: Episomal HIV-1 two-long terminal repeat (2-LTR) circles are considered markers for ongoing viral replication. Two sample processing procedures were compared to accurately quantify 2-LTR in patients by using droplet digital PCR (ddPCR). Here, we show that plasmid isolation with a spiked non-HIV plasmid for normalization enables more accurate 2-LTR quantification than genomic DNA isolation.

SUBMITTER: Malatinkova E

PROVIDER: S-EPMC4298544 | biostudies-literature | 2015 Feb

REPOSITORIES: biostudies-literature

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Accurate quantification of episomal HIV-1 two-long terminal repeat circles by use of optimized DNA isolation and droplet digital PCR.

Malatinkova Eva E Kiselinova Maja M Bonczkowski Pawel P Trypsteen Wim W Messiaen Peter P Vermeire Jolien J Verhasselt Bruno B Vervisch Karen K Vandekerckhove Linos L De Spiegelaere Ward W

Journal of clinical microbiology 20141210 2

Episomal HIV-1 two-long terminal repeat (2-LTR) circles are considered markers for ongoing viral replication. Two sample processing procedures were compared to accurately quantify 2-LTR in patients by using droplet digital PCR (ddPCR). Here, we show that plasmid isolation with a spiked non-HIV plasmid for normalization enables more accurate 2-LTR quantification than genomic DNA isolation. ...[more]

PMID: 25502524

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