Ontology highlight
ABSTRACT:
SUBMITTER: Malatinkova E
PROVIDER: S-EPMC4298544 | biostudies-literature | 2015 Feb
REPOSITORIES: biostudies-literature
Journal of clinical microbiology 20141210 2
Episomal HIV-1 two-long terminal repeat (2-LTR) circles are considered markers for ongoing viral replication. Two sample processing procedures were compared to accurately quantify 2-LTR in patients by using droplet digital PCR (ddPCR). Here, we show that plasmid isolation with a spiked non-HIV plasmid for normalization enables more accurate 2-LTR quantification than genomic DNA isolation. ...[more]