Dataset Information

Short-read, high-throughput sequencing technology for STR genotyping.

ABSTRACT: DNA-based methods for human identification principally rely upon genotyping of short tandem repeat (STR) loci. Electrophoretic-based techniques for variable-length classification of STRs are universally utilized, but are limited in that they have relatively low throughput and do not yield nucleotide sequence information. High-throughput sequencing technology may provide a more powerful instrument for human identification, but is not currently validated for forensic casework. Here, we present a systematic method to perform high-throughput genotyping analysis of the Combined DNA Index System (CODIS) STR loci using short-read (150 bp) massively parallel sequencing technology. Open source reference alignment tools were optimized to evaluate PCR-amplified STR loci using a custom designed STR genome reference. Evaluation of this approach demonstrated that the 13 CODIS STR loci and amelogenin (AMEL) locus could be accurately called from individual and mixture samples. Sensitivity analysis showed that as few as 18,500 reads, aligned to an in silico referenced genome, were required to genotype an individual (>99% confidence) for the CODIS loci. The power of this technology was further demonstrated by identification of variant alleles containing single nucleotide polymorphisms (SNPs) and the development of quantitative measurements (reads) for resolving mixed samples.

SUBMITTER: Bornman DM

PROVIDER: S-EPMC4301848 | biostudies-literature | 2012 Apr

REPOSITORIES: biostudies-literature

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Short-read, high-throughput sequencing technology for STR genotyping.

Bornman Daniel M DM Hester Mark E ME Schuetter Jared M JM Kasoji Manjula D MD Minard-Smith Angela A Barden Curt A CA Nelson Scott C SC Godbold Gene D GD Baker Christine H CH Yang Boyu B Walther Jacquelyn E JE Tornes Ivan E IE Yan Pearlly S PS Rodriguez Benjamin B Bundschuh Ralf R Dickens Michael L ML Young Brian A BA Faith Seth A SA

BioTechniques. Rapid dispatches 20120401

DNA-based methods for human identification principally rely upon genotyping of short tandem repeat (STR) loci. Electrophoretic-based techniques for variable-length classification of STRs are universally utilized, but are limited in that they have relatively low throughput and do not yield nucleotide sequence information. High-throughput sequencing technology may provide a more powerful instrument for human identification, but is not currently validated for forensic casework. Here, we present a s ...[more]

PMID: 25621315

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Dataset Information

Short-read, high-throughput sequencing technology for STR genotyping.

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Short-read, high-throughput sequencing technology for STR genotyping.

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