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3D-DIP-Chip: a microarray-based method to measure genomic DNA damage.


ABSTRACT: Genotoxins cause DNA damage, which can result in genomic instability. The genetic changes induced have far-reaching consequences, often leading to diseases such as cancer. A wide range of genotoxins exists, including radiations and chemicals found naturally in the environment, and in man-made forms created by human activity across a variety of industries. Genomic technologies offer the possibility of unravelling the mechanisms of genotoxicity, including the repair of genetic damage, enhancing our ability to develop, test and safely use existing and novel materials. We have developed 3D-DIP-Chip, a microarray-based method to measure the prevalence of genomic genotoxin-induced DNA damage. We demonstrate the measurement of both physical and chemical induced DNA damage spectra, integrating the analysis of these with the associated changes in histone acetylation induced in the epigenome. We discuss the application of the method in the context of basic and translational sciences.

SUBMITTER: Powell JR 

PROVIDER: S-EPMC4302307 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature

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3D-DIP-Chip: a microarray-based method to measure genomic DNA damage.

Powell James Rees JR   Bennett Mark Richard MR   Evans Katie Ellen KE   Yu Shirong S   Webster Richard Michael RM   Waters Raymond R   Skinner Nigel N   Reed Simon Huw SH  

Scientific reports 20150122


Genotoxins cause DNA damage, which can result in genomic instability. The genetic changes induced have far-reaching consequences, often leading to diseases such as cancer. A wide range of genotoxins exists, including radiations and chemicals found naturally in the environment, and in man-made forms created by human activity across a variety of industries. Genomic technologies offer the possibility of unravelling the mechanisms of genotoxicity, including the repair of genetic damage, enhancing ou  ...[more]

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