A Fluorescent Probe to Measure DNA Damage and Repair.
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ABSTRACT:
SUBMITTER: Condie AG
PROVIDER: S-EPMC4550365 | biostudies-literature | 2015
REPOSITORIES: biostudies-literature
Publications
A Fluorescent Probe to Measure DNA Damage and Repair.
Condie Allison G AG Yan Yan Y Gerson Stanton L SL Wang Yanming Y
PloS one 20150826 8
DNA damage and repair is a fundamental process that plays an important role in cancer treatment. Base excision repair (BER) is a major repair pathway that often leads to drug resistance in DNA-targeted cancer chemotherapy. In order to measure BER, we have developed a near infrared (NIR) fluorescent probe. This probe binds to a key intermediate, termed apurinic/apyrimidinic (AP) site, in the BER pathway where DNA damage and repair occurs. We have developed an assay to show the efficacy of the pro ...[more]
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