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High-Speed Coherent Raman Fingerprint Imaging of Biological Tissues.

ABSTRACT: An imaging platform based on broadband coherent anti-Stokes Raman scattering (BCARS) has been developed which provides an advantageous combination of speed, sensitivity and spectral breadth. The system utilizes a configuration of laser sources that probes the entire biologically-relevant Raman window (500 cm-1 to 3500 cm-1) with high resolution (< 10 cm-1). It strongly and efficiently stimulates Raman transitions within the typically weak "fingerprint" region using intrapulse 3-colour excitation, and utilizes the nonresonant background (NRB) to heterodyne amplify weak Raman signals. We demonstrate high-speed chemical imaging in two- and three-dimensional views of healthy murine liver and pancreas tissues and interfaces between xenograft brain tumours and the surrounding healthy brain matter.

SUBMITTER: Camp CH

PROVIDER: S-EPMC4304702 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature

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High-Speed Coherent Raman Fingerprint Imaging of Biological Tissues.

Camp Charles H CH Lee Young Jong YJ Heddleston John M JM Hartshorn Christopher M CM Hight Walker Angela R AR Rich Jeremy N JN Lathia Justin D JD Cicerone Marcus T MT

Nature photonics 20140101

An imaging platform based on broadband coherent anti-Stokes Raman scattering (BCARS) has been developed which provides an advantageous combination of speed, sensitivity and spectral breadth. The system utilizes a configuration of laser sources that probes the entire biologically-relevant Raman window (500 cm-1 to 3500 cm-1) with high resolution (< 10 cm-1). It strongly and efficiently stimulates Raman transitions within the typically weak "fingerprint" region usi ...[more]

PMID: 25621002

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