Nutrient-dependent secretion of glucose-dependent insulinotropic polypeptide from primary murine K cells.

ABSTRACT:

Aims/hypothesis

Glucose-dependent insulinotropic polypeptide (GIP) is an incretin hormone with anti-apoptotic effects on the pancreatic beta cell. The aim of this study was to generate transgenic mice with fluorescently labelled GIP-secreting K cells and to use these to investigate pathways by which K cells detect nutrients.

Methods

Transgenic mice were generated in which the GIP promoter drives the expression of the yellow fluorescent protein Venus. Fluorescent cells were purified by flow cytometry and analysed by quantitative RT-PCR. GIP secretion was assayed in primary cultures of small intestine.

Results

Expression of Venus in transgenic mice was restricted to K cells, as assessed by immunofluorescence and measurements of the Gip mRNA and GIP protein contents of p

SUBMITTER: Parker HE 

PROVIDER: S-EPMC4308617 | biostudies-literature | 2009 Feb

REPOSITORIES: biostudies-literature