Ontology highlight
ABSTRACT:
SUBMITTER: Tikhonova EB
PROVIDER: S-EPMC4308713 | biostudies-literature | 2015 Jan
REPOSITORIES: biostudies-literature
Tikhonova Elena B EB Ethayathulla Abdul S AS Su Yue Y Hariharan Parameswaran P Xie Shicong S Guan Lan L
Scientific reports 20150128
A highly diverse DNA library coding for ankyrin seven-repeat proteins (ANK-N5C) was designed and constructed by a PCR-based combinatorial assembly strategy. A bacterial melibiose fermentation assay was adapted for in vivo functional screen. We isolated a transcription blocker that completely inhibits the melibiose-dependent expression of α-galactosidase (MelA) and melibiose permease (MelB) of Escherichia coli by specifically preventing activation of the melAB operon. High-resolution crystal stru ...[more]