Ontology highlight
ABSTRACT:
SUBMITTER: Nishizawa-Yokoi A
PROVIDER: S-EPMC4309413 | biostudies-literature | 2015 Jan
REPOSITORIES: biostudies-literature
The Plant journal : for cell and molecular biology 20141110 1
Precise genome engineering via homologous recombination (HR)-mediated gene targeting (GT) has become an essential tool in molecular breeding as well as in basic plant science. As HR-mediated GT is an extremely rare event, positive-negative selection has been used extensively in flowering plants to isolate cells in which GT has occurred. In order to utilize GT as a methodology for precision mutagenesis, the positive selectable marker gene should be completely eliminated from the GT locus. Here, w ...[more]